This study has investigated the relationship in the connectivity of motor units in surface electromyogram (sEMG) of biceps brachii muscle. It is hypothesized that with ageing, there is reduction/loss in number of motor units, leading to reduction in the independence between the channels of the recorded muscle activity. Two channels of sEMG were recorded during three levels of isometric muscle contraction: 50 %, 75 % and 100 % maximal voluntary contraction (MVC). 73 subjects (age range 20-70)participated in the experiments. The independence in channel index (ICI) between the two sEMG recording locations was computed using the independent components and Frobenius norm. ANOVA Statistical analysis was performed to test the effect of age (loss of motor units) and level of contraction on ICI. The results show that the ICI among the older cohort was significantly lower compared with the younger adults. This research study has shown that the reduction in number of motor units is reflected by the reduction in the ICI of the sEMG signal.